Embedding, Microtomy, and Staining

HSTO 104

Explains the embedding of tissue processed by paraffin, factors in the choice of paraffin, melting points and influence on support. Students review the ultrastructure of the cell and the differences between nuclear and cytoplasmic staining. In addition, topics include theory of staining and impregnation, compassion of natural and synthetic dyes, examine types and uses for hematoxylin and differentiation between progression, regressive and polychromatic staining. Troubleshooting issues with Hematoxylin and Eosin (H&E) staining and contrast resinous media versus aqueous media will be explored. Course offered once a year (fall or spring).

  • Prerequisite(s) : HSTO 101 and HSTO 102
  • Credits: 3
  • Cost: $122/billable hour + fees (in county)
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